In vitro reconstitution of SARS-coronavirus mRNA cap methylation.
Identifieur interne : 000217 ( France/Analysis ); précédent : 000216; suivant : 000218In vitro reconstitution of SARS-coronavirus mRNA cap methylation.
Auteurs : Mickaël Bouvet [France] ; Claire Debarnot ; Isabelle Imbert ; Barbara Selisko ; Eric J. Snijder ; Bruno Canard ; Etienne DecrolySource :
- PLoS pathogens [ 1553-7374 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN messager, Coiffes des ARN (), Coiffes des ARN (génétique), Coiffes des ARN (métabolisme), Exoribonucleases (), Exoribonucleases (génétique), Exoribonucleases (métabolisme), Méthylation, Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Régulation de l'expression des gènes viraux, T-RNA methyltransferases, Techniques in vitro, Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Coiffes des ARN, Exoribonucleases, Protéines virales non structurales, Virus du SRAS.
- métabolisme : Coiffes des ARN, Exoribonucleases, Protéines virales non structurales, Virus du SRAS.
- ARN messager, Coiffes des ARN, Exoribonucleases, Méthylation, Protéines virales non structurales, Régulation de l'expression des gènes viraux, T-RNA methyltransferases, Techniques in vitro, Virus du SRAS.
English descriptors
- KwdEn :
- Exoribonucleases (chemistry), Exoribonucleases (genetics), Exoribonucleases (metabolism), Gene Expression Regulation, Viral, In Vitro Techniques, Methylation, RNA Caps (chemistry), RNA Caps (genetics), RNA Caps (metabolism), RNA, Messenger, SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (metabolism), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), tRNA Methyltransferases.
- MESH :
- chemical , chemistry : Exoribonucleases, RNA Caps, Viral Nonstructural Proteins.
- chemical , genetics : Exoribonucleases, RNA Caps, Viral Nonstructural Proteins.
- chemical , metabolism : Exoribonucleases, RNA Caps, Viral Nonstructural Proteins.
- chemistry : SARS Virus.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Gene Expression Regulation, Viral, In Vitro Techniques, Methylation, RNA, Messenger, tRNA Methyltransferases.
Abstract
SARS-coronavirus (SARS-CoV) genome expression depends on the synthesis of a set of mRNAs, which presumably are capped at their 5' end and direct the synthesis of all viral proteins in the infected cell. Sixteen viral non-structural proteins (nsp1 to nsp16) constitute an unusually large replicase complex, which includes two methyltransferases putatively involved in viral mRNA cap formation. The S-adenosyl-L-methionine (AdoMet)-dependent (guanine-N7)-methyltransferase (N7-MTase) activity was recently attributed to nsp14, whereas nsp16 has been predicted to be the AdoMet-dependent (nucleoside-2'O)-methyltransferase. Here, we have reconstituted complete SARS-CoV mRNA cap methylation in vitro. We show that mRNA cap methylation requires a third viral protein, nsp10, which acts as an essential trigger to complete RNA cap-1 formation. The obligate sequence of methylation events is initiated by nsp14, which first methylates capped RNA transcripts to generate cap-0 (7Me)GpppA-RNAs. The latter are then selectively 2'O-methylated by the 2'O-MTase nsp16 in complex with its activator nsp10 to give rise to cap-1 (7Me)GpppA(2'OMe)-RNAs. Furthermore, sensitive in vitro inhibition assays of both activities show that aurintricarboxylic acid, active in SARS-CoV infected cells, targets both MTases with IC(50) values in the micromolar range, providing a validated basis for anti-coronavirus drug design.
DOI: 10.1371/journal.ppat.1000863
PubMed: 20421945
Affiliations:
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pubmed:20421945Le document en format XML
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Sixteen viral non-structural proteins (nsp1 to nsp16) constitute an unusually large replicase complex, which includes two methyltransferases putatively involved in viral mRNA cap formation. The S-adenosyl-L-methionine (AdoMet)-dependent (guanine-N7)-methyltransferase (N7-MTase) activity was recently attributed to nsp14, whereas nsp16 has been predicted to be the AdoMet-dependent (nucleoside-2'O)-methyltransferase. Here, we have reconstituted complete SARS-CoV mRNA cap methylation in vitro. We show that mRNA cap methylation requires a third viral protein, nsp10, which acts as an essential trigger to complete RNA cap-1 formation. The obligate sequence of methylation events is initiated by nsp14, which first methylates capped RNA transcripts to generate cap-0 (7Me)GpppA-RNAs. The latter are then selectively 2'O-methylated by the 2'O-MTase nsp16 in complex with its activator nsp10 to give rise to cap-1 (7Me)GpppA(2'OMe)-RNAs. Furthermore, sensitive in vitro inhibition assays of both activities show that aurintricarboxylic acid, active in SARS-CoV infected cells, targets both MTases with IC(50) values in the micromolar range, providing a validated basis for anti-coronavirus drug design.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><noCountry><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Debarnot, Claire" sort="Debarnot, Claire" uniqKey="Debarnot C" first="Claire" last="Debarnot">Claire Debarnot</name><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name><name sortKey="Selisko, Barbara" sort="Selisko, Barbara" uniqKey="Selisko B" first="Barbara" last="Selisko">Barbara Selisko</name><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Bouvet, Mickael" sort="Bouvet, Mickael" uniqKey="Bouvet M" first="Mickaël" last="Bouvet">Mickaël Bouvet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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